Resources – Grainger Lab

PCR based mutagenesis strategies

FeBABE footprinting

RNAP alpha subunit purification

P1 Transduction

Chloroquine Gel Analysis

Curved DNA Binding Protein A purification

Conjugation

Western blotting

CRP purification

Plasmid maps in PowerPoint

Calcium Cholride competent E. coli

ChIP-seq protocols

End labelling DNA with gamma 32P-ATP

Fis purification

G+A ladder protocol

Glutaraldehyde crosslinking

H-NS purification

Identification of mRNA 5′ end by primer extension

In vitro transcription assays

LacZ assays

Phenol extraction and ethanol precipitation

Potassium Permanganate footprinting

RNAP sigma factor purification

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