PCR based mutagenesis strategies

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FeBABE footprinting

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RNAP alpha subunit purification

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P1 Transduction

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Chloroquine Gel Analysis

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Curved DNA Binding Protein A purification

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Conjugation

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Western blotting

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CRP purification

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Plasmid maps in PowerPoint

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Calcium Cholride competent E. coli

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ChIP-seq protocols

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End labelling DNA with gamma 32P-ATP

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Fis purification

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G+A ladder protocol

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Glutaraldehyde crosslinking

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H-NS purification

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Identification of mRNA 5′ end by primer extension

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In vitro transcription assays

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LacZ assays

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Phenol extraction and ethanol precipitation

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Potassium Permanganate footprinting

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RNAP sigma factor purification

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