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PCR based mutagenesis strategies
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FeBABE footprinting
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RNAP alpha subunit purification
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P1 Transduction
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Chloroquine Gel Analysis
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Curved DNA Binding Protein A purification
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Conjugation
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Western blotting
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CRP purification
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Plasmid maps in PowerPoint
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Calcium Cholride competent
E. coli
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ChIP-seq protocols
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End labelling DNA with gamma 32P-ATP
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Fis purification
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G+A ladder protocol
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Glutaraldehyde crosslinking
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H-NS purification
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Identification of mRNA 5′ end by primer extension
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In vitro transcription assays
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LacZ assays
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Phenol extraction and ethanol precipitation
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Potassium Permanganate footprinting
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RNAP sigma factor purification
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